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Product Name FENS1 Chinese Name 磷酸肌醇Binding protein1抗体 Alias FENS 1; FENS-1; KIAA1435; Phosphoinositide binding protein 1; Phosphoinositide binding protein SR1; Phosphoinositide-binding protein 1; WD repeat and FYVE domain containing 1; WD repeat and FYVE domain-containing protein 1; WD40 and FYVE domain containing protein 1; WD40- and FYVE domain-containing protein 1; WDF1; WDFY1; WDFY1_HUMAN; ZFYVE17; Zinc finger FYVE domain containing protein 17; Zinc finger FYVE domain-containing protein 17. literatures Specific References (2) | SL13169R has been referenced in 2 publications.[IF=7.561] Alexandru Tatomir. et al. RGC-32 Acts as a Hub to Regulate the Transcriptomic Changes Associated With Astrocyte Development and Reactive Astrocytosis. Front Immunol. 2021; 12: 705308 IHC ; Mouse.[IF=3.921] Anna Sancho-Balsells et al. Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1. Front Cell Neurosci. 2020 May 14;14:93. WB ; Mouse.Research Area Cell biology Signal transduction Transporter Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 46kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FENS1: 21-120/410 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDFY1 (WD repeat and FYVE domain containing 1), also known as WDF1, FENS-1 or ZFYVE17, is a 410 amino acid protein that localizes to the early endosome and contains one FYVE-type zinc finger and seven WD repeats through which it may play a role in protein trafficking and signal transduction.
Subunit:
Binds PtdIns3P in vitro with high specificity over other phosphoinositides.
Subcellular Location:
Early endosome.
Similarity:
Contains 1 FYVE-type zinc finger.
Contains 7 WD repeats.
SWISS:
Q8IWB7
Gene ID:
57590
Database links:Entrez Gene: 57590 Human
Entrez Gene: 69368 Mouse
SwissProt: Q8IWB7 Human
Unigene: 368359 Human
Unigene: 8557 Rat
Product Picture Sample:
Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug
Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 3: Skin (Rat) Tissue Lysate at 40 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: THP-1 (Human) Cell Lysate at 30 ug
Primary: Anti-FENS1 (SL13169R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: U2os (Human) Cell Lysate at 30 ug
Lane 4: A431 (Human) Cell Lysate at 30 ug
Primary:
Anti-FENS1 (SL13169R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FENS1) Polyclonal Antibody, Unconjugated (SL13169R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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