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Product Name EXOC2 Chinese Name 胞吐囊复合体蛋白2抗体 Alias exocyst complex component 2; exocyst complex component Sec5; FLJ11026; SEC5; SEC5 like 1; SEC5L1; Sec5p; EXOC2_HUMAN. Research Area Cell biology transcriptional regulatory factor Transporter Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 104kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human EXOC2/SEC5: 31-130/924 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The mammalian exocyst complex (sec 6/8) is a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Sec 5 is one of eight exocyst complex subunits: Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. Exocyst complex regulation depends upon Sec5 binding Ral in a GTP-dependent manner. Human Sec5 maps to chromosome 6p25.3.
Function:
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Subunit:
The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1 (By similarity). Interacts with RALA and GNEFR/DELGEF. Interaction with GNEFR occurs only in the presence of magnesium or manganese and is stimulated by dCTP or GTP. Interacts with RALB.
Subcellular Location:
Plasma membrane.
Tissue Specificity:
Widely expressed with highest levels in brain and placenta.
Similarity:
Belongs to the SEC5 family.
Contains 1 IPT/TIG domain.
SWISS:
Q96KP1
Gene ID:
55770
Database links:Entrez Gene: 55770 Human
Entrez Gene: 66482 Mouse
SwissProt: Q96KP1 Human
SwissProt: Q9D4H1 Mouse
Unigene: 484412 Human
Unigene: 293510 Mouse
Unigene: 2869 Rat
Product Picture Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(SL13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(SL13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC2) Polyclonal Antibody, Unconjugated (SL13118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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