Product Name	Epsin 2
Chinese Name	内吞作用辅助蛋白EPN2抗体
Alias	EHB21; EPN 2; EPN-2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889.
Research Area	Tumour  Cell biology  Growth factors and hormones  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	69kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Epsin 2: 31-130/641
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer. Two novel EGF receptor substrates have been described, designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15. Epsin is a binding partner to Eps15. Both epsin and Eps15 have an ubiquitous tissue distribution but are concentrated in presynaptic nerve terminals specialized for the Clathrin-mediated endocytosis of synaptic vesicles. Disruption of epsin function blocks Clathrin-mediated endocytosis. Epsin, along with its binding partner Eps15, is proposed to be involved in the assistance of Clathrin coat rearrangement during Clathrin coated pit invagination. Epsin 2a, and 2b are splicing variants of epsin 2, which is associated with Clathrin-mediated endocytosis and are enriched in the brain in the peri-Golgi region. Function: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Subunit: Binds EPS15 (By similarity). Binds AP-2 and clathrin. Subcellular Location: Cytoplasm. Cytoplasmic vesicle; clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex. Tissue Specificity: Highest expression is found in brain. Detected at lower levels in lung and liver. Post-translational modifications: Ubiquitinated. Similarity: Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 2 UIM (ubiquitin-interacting motif) repeats. SWISS: O95208 Gene ID: 22905 Database links: Entrez Gene: 22905 Human Entrez Gene: 13855 Mouse Entrez Gene: 60443 Rat Omim: 607263 Human SwissProt: O95208 Human SwissProt: Q8CHU3 Mouse SwissProt: Q9Z1Z3 Rat Unigene: 309467 Human Unigene: 139695 Mouse Unigene: 436466 Mouse Unigene: 44273 Rat
Product Picture	Sample: HepG2(human) Cell Lysate at 40 ug Primary: Anti-Epsin2(SL13102R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Epsin 2) Polyclonal Antibody, Unconjugated (SL13102R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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