TEL: +86 571 56623320 EMAIL: [email protected]
Product Name eRF1 Chinese Name 真核肽链释放因子1抗体 Alias Cl1 protein; D5S1995; ERF; eRF1; ERF1_HUMAN; ETF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Eukaryotic translation termination factor 1; MGC111066; Polypeptide chain release factor 1; Protein Cl1; RF1; Sup45 (yeast omnipotent suppressor 45) homolog like 1; SUP45L1; TB3 1; TB3-1. Research Area Cell biology transcriptional regulatory factor Epigenetics G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Zebrafish, Arabidopsis Thaliana, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 49kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human eRF1: 341-437/437 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012].
Function:
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Subunit:
Heterodimer of two subunits, one of which binds GTP. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the eukaryotic release factor 1 family.
SWISS:
P62495
Gene ID:
2107
Database links:Entrez Gene: 2107 Human
Omim: 600285 Human
SwissProt: P62495 Human
Unigene: 483494 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eRF1) Polyclonal Antibody, Unconjugated (SL13098R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode