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Product Name PDLIM2 Chinese Name PDLIM2蛋白抗体 Alias FLJ34715; MYSTIQUE; OTTHUMP00000162522; OTTHUMP00000205197; OTTHUMP00000205198; OTTHUMP00000205199; OTTHUMP00000205200; OTTHUMP00000205202; OTTHUMP00000205203; PDLI2_HUMAN; Pdlim2; PDZ and LIM domain 2 (mystique); PDZ and LIM domain protein 2; PDZ-LIM protein mystique; SLIM; Stat-interacting LIM protein. Research Area Tumour Cell biology Signal transduction Binding protein Cytoskeleton Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Horse, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 37kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PDLIM2: 31-130/352 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
Function:
Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.
Subcellular Location:
Cytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin.
Similarity:
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
SWISS:
Q96JY6
Gene ID:
64236
Database links:
Entrez Gene: 64236 Human
Entrez Gene: 213019 Mouse
Omim: 609722 Human
SwissProt: Q96JY6 Human
SwissProt: Q8R1G6 Mouse
Unigene: 632034 Human
Unigene: 283968 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDLIM2) Polyclonal Antibody, Unconjugated (SL12600R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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