Product Name	FLRT2
Chinese Name	富含亮氨酸跨膜纤连蛋白2抗体
Alias	Fibronectin leucine rich transmembrane protein 2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; KIAA0405; Leucine rich repeat transmembrane protein; Leucine rich repeat transmembrane protein FLRT2; FLRT2_HUMAN.
Research Area	Cell biology  Developmental biology  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	70kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FLRT2: 201-300/660 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	FLRT2 is a 660 amino acid single-pass type I membrane protein that is expressed in pancreas, skeletal muscle, brain and heart. Comprised of one fibronectin type-III domain and ten LRR (leucine-rich) repeats, FLRT2 may play a role in cell adhesion and/or receptor signaling. It is suggested that FLRT2 is involved in mediating events such as NCC (neural crest cell) migration, chondrogenesis and epithelial-mesenchymal interactions during craniofacial development. Function: May have a function in cell adhesion and/or receptor signaling. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in pancreas, skeletal muscle, brain, and heart. Post-translational modifications: N-glycosylated. Similarity: Contains 1 fibronectin type-III domain. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: O43155 Gene ID: 23768 Database links: Entrez Gene: 23768 Human Entrez Gene: 399558 Mouse Omim: 604807 Human SwissProt: O43155 Human Unigene: 533710 Human Unigene: 341948 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLRT2) Polyclonal Antibody, Unconjugated (SL12561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FLRT2 Polyclonal Antibody, Unconjugated(SL12561R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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