Product Name	Apolipoprotein D
Chinese Name	载LipoproteinD抗体
Alias	Apolipoprotein D; APOD; APO D; APOD protein; APOD_HUMAN; Apolipoprotein D; Apo-D; ApoD.
Research Area	Tumour  Cardiovascular  Cell biology  immunology  Neurobiology  Alzheimer's
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	19kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human APOD: 81-189/189
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Apolipoprotein D (APO D), a glycoprotein involved in the human plasma lipid transport system. It is a progesterone binding glycoprotein of 24,000 dalton monomer molecular size, is a constituent of high density lipoprotein in plasma. The function of apolipoprotein D in the metabolism of plasma lipoproteins is unclear but the observation that this protein forms complexes with lecithin: cholesterol acyltransferase has led to the suggestion that apolipoprotein D may be involved in cholesterol esterification and transport of substrates and products of the reaction. Apolipoprotein D is expressed in a range of normal tissues including axillary apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves, pituitary, testis, cerebellum and renal tubes are also positive. APO D was first isolated in large quantity as GCDFP 24, the major protein component of most human breast cyst fluids. In recent studies, Apo D immunoreactivity has been shown to be an early indicator of prostate cancer and advanced primary prostate tumors. Function: APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Subunit: Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2. Subcellular Location: Secreted. Tissue Specificity: Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Post-translational modifications: N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor). Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P05090 Gene ID: 347 Database links: Entrez Gene: 347 Human Omim: 107740 Human SwissProt: P05090 Human Unigene: 522555 Human 载LipoproteinD(apolipoprotein D,apoD)是一个glycoprotein,它最初是在人血浆的高密度Lipoprotein中被分离出来的。apoD在结构上与其他类型的载Lipoprotein存在很大的差异,被归入脂肪促成素家族。apoD可以与胆固醇、黄体酮、胆红素等多种疏水性小分子结合。 apoD在多种脊索动物的各类组织中广泛表达, 在脊椎动物中重要的生理功能。最近的研究表明,apoD可以作为多种癌症及神经系统疾病的早期诊断标记, apoD是一个多配体、多功能的蛋白质.
Product Picture	Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Apolipoprotein D (SL1254R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 30 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-APOD Polyclonal Antibody, Unconjugated(SL1254R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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