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Product Name API5 Chinese Name 凋亡抑制因子5抗体 Alias apoptosis inhibitor 5; apoptosis inhibitory protein 5; API5; AAC-11; AAC11; API5L1; AAC 11; Antiapoptosis clone 11 protein; API5 like 1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; Migration inducing protein MIG8; MIG 8; MIG8; Protein XAGL; API5_HUMAN. Research Area Tumour immunology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Guinea Pig, ) Applications WB=1:500-2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human API5: 351-460/504 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. API5 expression is often upregulated in tumor cells, particularly in metastatic cells. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription. Alternate Names: AAC 11; AAC11; Antiapoptosis clone 11 protein; API 5; API5; API5 like 1; API5L1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL.
Function:
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Subunit:
Monomer. Interacts with FGF2 and ACIN1.
Subcellular Location:
Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic.
Isoform 3: Cytoplasm.
Tissue Specificity:
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
Post-translational modifications:
Acetylation at Lys-251 impairs antiapoptotic function.
Similarity:
Belongs to the API5 family.
SWISS:
Q9BZZ5
Gene ID:
8539
Database links:Entrez Gene: 8539 Human
Entrez Gene: 11800 Mouse
Omim: 609774 Human
SwissProt: Q9BZZ5 Human
SwissProt: O35841 Mouse
Unigene: 435771 Human
Unigene: 181824 Mouse
Unigene: 402557 Mouse
IAP-5是Apoptosis的一种抑制因子、参与Apoptosis调控,可以用于各种恶性Tumour的Apoptosis的研究.Product Picture Sample:
Heart (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-API5 (SL1252R) at 1:300
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1:5000 dilution
Predicted band size : 57 kD
Observed band size :57 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (SL1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (API5) Polyclonal Antibody, Unconjugated (SL1252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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