Product Name	CD166
Chinese Name	活化白Cell adhesion molecule抗体
Alias	activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN.
literatures	Specific References  (10)     |     SL1251R has been referenced in 10 publications. [IF=6.081] Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936  ICC ;  Human.   PubMed:37283947 [IF=4.486] Wenping Luo. et al. BMP9‐initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/β‐catenin signalling activity. 2021 Feb 18  IF ;  Mouse.   PubMed:33605035 [IF=4.175] Yue Wu. et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int. 2021 Dec;21(1):1-14  FC ;  Human.   PubMed:34051810 [IF=3.759] Jaromír Vašíček. et al. Molecular Profiling and Gene Banking of Rabbit EPCs Derived from Two Biological Sources. Genes-Basel. 2021 Mar;12(3):366  FC ;  Rabbit.   PubMed:33806502 [IF=3.739] Vašíček J et al. Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. N Biotechnol. 2019 Aug 7;54:1-12.  FCM ;  Rabbit.   PubMed:31400479 [IF=3.53] Lee, Tao-Chen, et al. "Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells." PLOS ONE 9.11 (2014): e111390.  Rabbit.   PubMed:25380245 [IF=3.231] Zhen Zhou. et al. Myogenic Determination and Differentiation of Chicken Bone Marrow-Derived Mesenchymal Stem Cells under Different Inductive Agents. ANIMALS. 2022 Jan;12(12):1531  IF ;  Chicken.   PubMed:35739868 [IF=2.784] Ma C et al.Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos.Int J Mol Med. 2018 Jul;42(1):525-533.  ICF ;  Sheep.   PubMed:29693133 [IF=2.634] Ma C et al. Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove. DNA Cell Biol. 2018 Sep;37(9):731-741.  ICF ;  Goose.   PubMed:30102556 [IF=1.99] Kovac, Michal, et al. "Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells." Biotechnology Progress (2017).  FCM ;  Rabbit.   PubMed:28653478
Research Area	Tumour  Cell adhesion molecule  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	62kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD166: 451-583/583 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166. The expression of CD166 is up-regulated in low-grade prostate tumors and down-regulated in high-grade tumors; may play role in progression of prostate cancer. Function: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and hemophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: Q13740 Gene ID: 214 Database links: Entrez Gene: 214 Human Entrez Gene: 11658 Mouse Entrez Gene: 79559 Rat Omim: 601662 Human SwissProt: Q13740 Human SwissProt: Q61490 Mouse SwissProt: O35112 Rat Unigene: 591293 Human Unigene: 288282 Mouse Unigene: 5789 Rat ALCAM/CD166是免疫球蛋白超家族的成员,对多种Tumour生长和转移相关的Tumour细胞特性具有调节作用,ALCAM/CD166在乳腺癌、前列腺癌、食道癌、结肠癌及恶性黑色素瘤等Tumour都有一定的表达，尤以结肠、直肠癌表达显著。
Product Picture	Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-CD166 (SL1251R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62/70 kD Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-CD166 (SL1251R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62/70 kD Blank control: Molt-4 Cells(blue). Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody (SL1251R/AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (SL1251R/AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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