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Product Name ARHGAP15 Chinese Name Rho GTP酶激活蛋白15抗体 Alias ArhGAP15; BM046; Rho GTPase activating protein 15; Rho type GTPase activating protein 15; RHG15_HUMAN. Research Area Cell biology Signal transduction G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ARHGAP15: 201-300/861 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Rho GTPases, which play fundamental roles in numerous cellular processes, are initiated by external stimuli that signal though G-protein coupled receptors. ARHGEF15 (Rho guanine nucleotide exchange factor (GEF) 15), also known as ARGEF15 or Vsm-RhoGEF, is a 841 amino acid protein expressed in the vascular smooth muscle of coronary artery. ARHGEF15 functions as a specific guanine nucleotide exchange factor for RhoA and interacts with ephrin-A4 in vascular smooth muscle cells. Containing one DH (DBL-homology) domain, ARHGEF15 is phosphorylated on tyrosine residues upon ephrin-A1 stimulation. The DH domain consists of a region of about 150 amino acids that induces Rho family GTPases to release GDP. This effectively activates the Rho GTPase by allowing GTP binding. ARHGEF15 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
Function:
ARHGAP15 is an activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Overexpression results in an increase in actin stress fibers and cell contraction.
Subcellular Location:
Cytoplasm. Membrane; Peripheral membrane protein
Similarity:
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
SWISS:
Q53QZ3
Gene ID:
55843
Database links:Entrez Gene: 55843 Human
Omim: 610578 Human
SwissProt: Q53QZ3 Human
Unigene: 171011 Human
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