Product Name	APPBP1
Chinese Name	β淀粉样蛋白前体蛋白Binding protein1抗体
Alias	59 kDa; A 116A10.1; Amyloid beta precursor protein binding protein 1 59kD; Amyloid beta precursor protein binding protein 1 59kDa; Amyloid beta precursor protein binding protein 1; Amyloid beta precursor protein-binding protein 1; Amyloid protein binding protein 1; Amyloid protein-binding protein 1; APP BP1; APP-BP1; APPBP 1; HPP 1; HPP1; NAE 1; NAE1; NEDD8 activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit 1; NEDD8 activating enzyme E1 subunit; NEDD8-activating enzyme E1 regulatory subunit; Proto oncogene protein 1; Proto-oncogene protein 1; Protooncogene protein 1; ula 1; ULA1_HUMAN.
literatures	Specific References  (1)     |     SL12503R has been referenced in 1 publications. [IF=2.1] Xiangdong Meng. et al. Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer's disease by regulating multiple metabolic pathways. Mol Med Rep. 2021 May;23(5):1-13  WB ;  Mouse.   PubMed:33760152
Research Area	Cell biology  Neurobiology  Epigenetics  Ubiquitin  The cell membrane蛋白  Alzheimer's
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human APPBP1: 401-500/534
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	APPBP1 is a member of the ubiquitin-activating E1 family. In fetal tissues APPBP1 is widely expressed and in adult tissues it is expressed throughout the brain. APPBP1 is a cell membrane associated protein and functions as the regulatory subunit in a heterodimer with UBA3. The APPBP1/UBA3 complex binds to and activates NEDD8, a ubiquitin-like protein involved in signal transduction, cell proliferation and development. This suggests that APPBP1 affects a variety of cellular functions. In addition, APPBP1 is essential for cell cycle progression through the S/M checkpoint. More specifically, it inhibits the entry into S phase and promotes entry into M phase. Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Subunit: Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2. Subcellular Location: Cell membrane. Colocalizes with APP in lipid rafts. Tissue Specificity: Ubiquitous in fetal tissues. Expressed throughout the adult brain. Post-translational modifications: Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Similarity: Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. SWISS: Q13564 Gene ID: 8883 Database links: Entrez Gene: 8883 Human Entrez Gene: 234664 Mouse Omim: 603385 Human SwissProt: Q13564 Human SwissProt: Q8VBW6 Mouse Unigene: 460978 Human Unigene: 237670 Mouse
Product Picture	Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-APPBP1 (SL12503R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (SL12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (SL12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APPBP1) Polyclonal Antibody, Unconjugated (SL12503R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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