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Product Name S100B Chinese Name S100B蛋白抗体 Alias S100 beta; NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta (neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta. S100 β; S100-β; S100β; literatures Specific References (3) | SL1248R has been referenced in 3 publications.[IF=8.025] Kai-Chiang Yang. et al. Bioinspired collagen-gelatin-hyaluronic acid-chondroitin sulfate tetra-copolymer scaffold biomimicking native cartilage extracellular matrix facilitates chondrogenesis of human synovium-derived stem cells. INT J BIOL MACROMOL. 2023 Apr;:124400 IHC ; Mouse.[IF=4.959] Yang KC et al. Effects of scaffold geometry on chondrogenic differentiation of adipose-derived stem cells. Materials Science and Engineering: C, 110733. IHC-P ; Rabbit.[IF=3.857] Chen-Chie Wang. et al. Infrapatellar Fat Pads–Derived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold:. Cartilage. 0;(): IHC ; Rabbit.Research Area Tumour Cell biology immunology Neurobiology Signal transduction Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Cow, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 10kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human S100B: 2-50/92 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Function:
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Similarity:
Belongs to the S-101 family.
Contains 2 EF-hand domains.
SWISS:
P04271
Gene ID:
6285
Database links:Entrez Gene: 6285 Human
Entrez Gene: 20203 Mouse
Omim: 176990 Human
SwissProt: P04271 Human
SwissProt: P50114 Mouse
Unigene: 422181 Human
Unigene: 235998 Mouse
Unigene: 8937 Rat
S100B是一组可溶性、相对低分子质量、高酸性蛋白,属神经胶质细胞特异性蛋白,亦可存在于神经纤维中。在研究肠神经系统的形态特征及结构特征中,S100B有良好的表达功能。
S100B蛋白其生理作用尚不清楚,可能与细胞内钙离子媒介的调节作用有关。S100B蛋白可存在于神经鞘瘤,成软骨细胞瘤、软骨肉瘤、骨肉瘤、神经胶质瘤、郎格罕斯细胞瘤、一些癌肿和黑素细胞Tumour等。
在免疫组化中,除了恶性黑素瘤皮损,S100B蛋白还在良性黑素细胞痣中增加,如:蓝痣、普通后天痣、雀斑样痣、Spitz痣、气球状细胞痣、色素性梭形细胞痣和发育不良痣中表达。还有报道S100B蛋白的染色强度与Tumour细胞的黑素含量呈负相关。结缔组织增生性的或亲神经的黑素瘤不易染色。
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Polyclonal Antibody, Unconjugated (SL1248R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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