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Product Name phospho-APC1 (Ser688) Chinese Name 磷酸化细胞周期末期促进复合蛋白APC1抗体 Alias phospho-ANAPC1(Ser688); Apc1 (phospho S688); ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specific gene 24 protein; TSG 24; TSG24. Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 215kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human APC1 around the phosphorylation site of Ser688: SL(p-S)PV Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011].
Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Subunit:
The APC/C is composed of at least 12 subunits.
Post-translational modifications:
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
Similarity:
Belongs to the APC1 family.
Contains 4 PC repeats.
SWISS:
Q9H1A4
Gene ID:
64682
Database links:Entrez Gene: 64682 Human
Entrez Gene: 17222 Mouse
Omim: 608473 Human
SwissProt: Q9H1A4 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ANAPC) Polyclonal Antibody, Unconjugated (SL12482R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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