Product Name	Annexin A13
Chinese Name	膜粘连蛋白13抗体
Alias	Annexin A13; Annexin XIII; Annexin, intestine-specific; Annexin-13; ANX 13; ANX A13; ANX13; ANX13_HUMAN; ANXA13; Intestine Specific Annexin; Intestine-specific annexin; ISA; MGC150460.
Research Area	Cell biology  Signal transduction  Channel protein  Cell adhesion molecule  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Annexin A13: 241-316/316
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Annexins constitute a family of structurally-related, relatively abundant proteins that exhibit Ca2+-dependent binding to phospholipids. Annexins function in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, inhibition of phospholipase A2, inhibition of coagulation and mediation of cell-matrix interactions. Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function. Two alternative splicing isoforms of Annexin A13 exist, both of which bind to rafts. Subcellular Location: Cell membrane. Associated with the plasma membrane of undifferentiated, proliferating crypt epithelial cells as well as differentiated villus enterocytes. Tissue Specificity: Gut specific. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P27216 Gene ID: 312 Database links: Entrez Gene: 312 Human Entrez Gene: 69787 Mouse Entrez Gene: 362915 Rat Omim: 602573 Human SwissProt: P27216 Human SwissProt: Q99JG3 Mouse Unigene: 181107 Human Unigene: 237985 Mouse Unigene: 100125 Rat
Product Picture	Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti- Annexin A13 (SL12478R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Annexin A13 Polyclonal Antibody, Unconjugated(SL12478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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