Product Name	HIC5
Chinese Name	转化生长因子β1诱导转录因子1抗体
Alias	Androgen receptor coactivator 55 kDa protein; Androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; Hic-5; Hydrogen peroxide-inducible clone 5 protein; TGFB1I1; TGFI1_HUMAN; Transforming growth factor beta 1 induced transcript 1; Transforming growth factor beta-1-induced transcript 1 protein; TSC 5.
Research Area	Cell biology  Chromatin and nuclear signals  Signal transduction  Stem cells  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TGFB1I1/HIC5: 31-130/461
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16. Function: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity. Subunit: Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7 (By similarity). Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Subcellular Location: Cell junction; focal adhesion. Nucleus matrix. Cytoplasm; cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers. Tissue Specificity: Expressed in platelets, smooth muscle and prostate stromal cells (at protein level). Post-translational modifications: Phosphorylated by gonadotropin-releasing hormone-activated SRC. Similarity: Belongs to the paxillin family. Contains 4 LIM zinc-binding domains. SWISS: O43294 Gene ID: 7041 Database links: Entrez Gene: 7041 Human Entrez Gene: 21804 Mouse Entrez Gene: 84574 Rat Entrez Gene: 515834 Cow Omim: 602353 Human SwissProt: Q3MHZ4 Cow SwissProt: O43294 Human SwissProt: Q9BPW3 Human SwissProt: Q3YBY7 Mouse SwissProt: Q3YBY8 Mouse SwissProt: Q3YBZ0 Mouse SwissProt: Q3YBZ1 Mouse SwissProt: Q3YBZ3 Mouse SwissProt: Q3YBZ4 Mouse SwissProt: Q3YBZ5 Mouse SwissProt: Q3YBZ6 Mouse SwissProt: Q62219 Mouse SwissProt: Q99PD6 Rat Unigene: 513530 Human Unigene: 3248 Mouse Unigene: 103260 Rat
Product Picture	Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HIC5 Polyclonal Antibody, Unconjugated(SL12436R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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