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Product Name Caseine Kinase 1 alpha Chinese Name 酪蛋白激酶casein kinase Iα抗体 Alias Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975. Research Area Tumour Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 39kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CK1A/Caseine Kinase 1 alpha: 221-337/337 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Function:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Subunit:
Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation.
Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Note=Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.
Casein kinase I subfamily.
Contains 1 protein kinase domain.
SWISS:
P48729
Gene ID:
1452
Database links:Entrez Gene: 1452 Human
Entrez Gene: 93687 Mouse
Omim: 600505 Human
SwissProt: P48729 Human
SwissProt: Q8BK63 Mouse
Product Picture Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caseine Kinase 1 alpha Polyclonal Antibody, Unconjugated(SL12427R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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