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Product Name phospho-CSNK1G 1+2+3 ( Tyr263) Chinese Name 磷酸化酪蛋白激酶1γ1+γ2+γ3抗体 Alias Casein kinase I isoforms gamma 1+2+3 (phospho Y263); p-CK1g2(phospho Y263); p-CSNK1G1(phospho Y263); p-CSNK1G2(phospho Y263); p-CSNK1G3(phospho Y263); Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3; KC1G1_HUMAN. Product Type Phosphorylated anti Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 49kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Casein kinase I isoforms gamma 1+2+3 around the phosphorylation site of Tyr263: ER(p-Y)QK Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. They can phosphorylate a large number of proteins. Participates in Wnt signaling.
Function:
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.
Subunit:
Monomer.
Subcellular Location:
Cytoplasm.
Post-translational modifications:
Autophosphorylated (By similarity).
Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.
SWISS:
Q9HCP0
Gene ID:
53944
Database links:Entrez Gene: 53944 Human
Entrez Gene: 214897 Mouse
Omim: 606274 Human
SwissProt: Q9HCP0 Human
SwissProt: Q8BTH8 Mouse
Unigene: 646508 Human
Unigene: 325816 Mouse
Product Picture Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (SL12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (SL12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (SL12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CSNK1G 1+2+3 ( Tyr263)) Polyclonal Antibody, Unconjugated (SL12426R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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