Product Name	CAPRIN2
Chinese Name	胃癌多药耐药相关蛋白
Alias	C1q domain-containing protein 1; C1QDC1; CAPR2_HUMAN; Caprin family member 2; Caprin-2; caprin2; Cytoplasmic activation/proliferation-associated protein 2; EEG 1; EEG1; Gastric cancer multidrug resistance-associated protein; KIAA1873; Protein EEG-1; RNA granule protein 140; RNG140.
Research Area	Cell biology  Signal transduction  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Pig, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	126kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CAPRIN2: 101-200/1127
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caprin2 is 1127 amino acid highly conserved protein that is ubiquitously expressed with highest levels of expression in brain and spleen. Caprin2 stabilizes cytosolic β-catenin and enhances LEF-1 dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Caprin2 promotes LRP5/6 phosphorylation by GSK-3 and enhances the interaction between Axin and LRP5/6. It is suggested that Caprin2 functions as a proapoptotic inhibitor of the cell cycle. Nine isoforms of Caprin2 exist due to alternative splicing events. Function: May regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons. Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation. May be involved in apoptosis. Subcellular Location: Cytoplasm; Mitochondrion. Colocalizes with aggregated mitochondria and Mitochondrion. Cytoplasm. Expressed throughout the cytoplasm. Tissue Specificity: Detected in all tissues tested with highest levels of expression in brain and spleen. Similarity: Belongs to the caprin family. Contains 1 C1q domain. SWISS: Q6IMN6 Gene ID: 65981 Database links: Entrez Gene: 65981 Human Omim: 610375 Human SwissProt: Q6IMN6 Human Unigene: 234355 Human
Product Picture	Sample: U87MG(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug Primary: Anti- CAPRIN2 (SL12423R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 126 kD Observed band size: 126 kD Sample: Lane 1: U251 (Human) Cell Lysate at 30 ug Lane 2: U87MG (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Primary: Anti-CAPRIN2 (SL12423R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120-140 kD Observed band size: 140 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (SL12423R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (SL12423R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-CAPRIN2 antibody(SL12423R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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