Product Name	BAMBI
Chinese Name	Tumour转移抑制蛋白BAMBI抗体
Alias	BAMBI; BAMBI_HUMAN; BMP and activin membrane bound inhibitor homolog; BMP and activin membrane-bound inhibitor homolog; NMA; Non metastatic gene A protein; Non-metastatic gene A protein; Putative transmembrane protein NMA.
literatures	Specific References  (5)     |     SL12418R has been referenced in 5 publications. [IF=17.521] Hongwei Chen. et al. Dissecting Heterogeneity Reveals a Unique BAMBIhighMFGE8high Subpopulation of Human UC-MSCs. Advanced Science. 2022 Nov;:2202510  WB ;  Human.   PubMed:36373720 [IF=5.23] He, Yao, et al. "LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia." Scientific reports 6 (2016): 27051.  IHC-P, IF(ICC) ;  Human.   PubMed:27243216 [IF=2.812] Yaqi Zhang. et al. Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep. MOL REPROD DEV. 2023 Feb;:  IF,WB ;  Sheep.   PubMed:36775976 [IF=2.08] Bai, Long, et al. "Identification and Expression Analyses of BAMBI Mediated by FSH in Swine Luteinizing Granulosa Cells." Theriogenology (2014).  Pig.   PubMed:25168722 [IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.  WB ;  Mouse.   PubMed:26781175
Research Area	Tumour  Stem cells  Growth factors and hormones  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	27kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BAMBI: 101-200/260 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines. Function: Negatively regulates TGF-beta signaling. Subcellular Location: Membrane. Tissue Specificity: High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. Similarity: Belongs to the BAMBI family. SWISS: Q13145 Gene ID: 25805 Database links: Entrez Gene: 25805 Human Entrez Gene: 68010 Mouse Entrez Gene: 449001 Pig Entrez Gene: 83837 Rat Omim: 604444 Human SwissProt: Q13145 Human SwissProt: Q9D0L6 Mouse SwissProt: Q91XN4 Rat Unigene: 533336 Human Unigene: 284863 Mouse Unigene: 25267 Rat
Product Picture	Sample: RAW264.7 (Mouse) CellLysate at 30 ug Primary: Anti-BAMBI (SL12418R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Primary: Anti-BAMBI (SL12418R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 30 kD Sample: Testis (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-BAMBI (SL12418R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(SL12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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