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Product Name GCNT1 Chinese Name GCNT1葡萄糖转移酶抗体 Alias 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase; Beta-1; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase; C2GNT; C2GNT L; C2GNT1; C2GnTL; Core 2 branching enzyme; Core 2 GNT; Core 2-branching enzyme; Core2 GlcNAc transferase; Core2-GlcNAc-transferase; GCNT 1; GCNT 1; GCNT1; GCNT1_HUMAN; glucosaminyl (N-acetyl) transferase 1 core 2; NACGT2. Research Area Tumour Cell biology Signal transduction glycoprotein The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Human GCNT1: 201-260/428 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins.
Function:
Forms critical branches in O-glycans.
Subcellular Location:
Golgi apparatus membrane.
Tissue Specificity:
Highly expressed in activated T-lymphocytes and myeloid cells.
Similarity:
Belongs to the glycosyltransferase 14 family.
SWISS:
Q02742
Gene ID:
2650
Database links:Entrez Gene: 2650 Human
Entrez Gene: 14537 Mouse
Omim: 600391 Human
SwissProt: Q02742 Human
SwissProt: Q09324 Mouse
Unigene: 521568 Human
Unigene: 244825 Mouse
Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
U87MG (Human) Cell Lysate at 30 ug
Primary: Anti- GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human TT cell lysates
Lane 4: Human U87MG cell lysates
Lane 5: Human MCF-7 cell lysates
Primary: Anti-GCNT1 (SL12334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNT1) Polyclonal Antibody, Unconjugated (SL12334R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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