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Product Name Stella Chinese Name 发育多能性相关蛋白3抗体 Alias DPPA3; Compaction associated protein 1; Developmental pluripotency associated 3; Developmental pluripotency associated protein 3; Developmental pluripotency-associated protein 3; Dppa 3; Dppa3; DPPA3_HUMAN; PGC7; Stella related protein; Stella-related protein; Stellar. literatures Specific References (1) | SL12280R has been referenced in 1 publications.[IF=1.84] Wasielak, M., et al. "Zygote arrest 1 (ZAR-1), nucleoplasmin 2 (NPM2), and developmentally associated protein 3 (DPPA3) mRNA profiles throughout porcine embryo development in vitro." Theriogenology (2016). IHC-F ; Pig.Research Area Tumour Developmental biology Stem cells transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Stella/DPPA3: 101-159/159 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008]
Function:
May play a role in maintaining cell pluripotentiality.
Subcellular Location:
Nucleus. Cytoplasm.
Tissue Specificity:
Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
DISEASE:
Expressed in fetal ovary.
SWISS:
Q6W0C5
Gene ID:
359787
Database links:Entrez Gene: 359787 Human
Entrez Gene: 73708 Mouse
Omim: 608408 Human
SwissProt: Q6W0C5 Human
SwissProt: Q8QZY3 Mouse
Unigene: 131358 Human
Unigene: 528118 Human
Unigene: 27982 Mouse
Unigene: 79056 Rat
Product Picture Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Stella (SL12280R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Stella/DPPA3 Polyclonal Antibody, Unconjugated(SL12280R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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