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Product Name ZNF312 Chinese Name Zinc finger protein312抗体 Alias Fez; FEZ family zinc finger protein 2; FEZF 2; FEZF2; FEZL; FKSG36; FLJ10142; Forebrain embryonic zinc finger like protein 2; TOF; ZFP 312; Zfp312; Zinc finger FEZL; Zinc finger protein 312; Zinc finger protein FEZ like; ZNF 312; FEZF2_HUMAN. Research Area Cell biology Neurobiology Signal transduction transcriptional regulatory factor Zinc finger protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 49kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZNF312: 10-120/459 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons.
Function:
Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. May be involved in innate immunity (By similarity).
Subcellular Location:
Nuclear.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
SWISS:
Q8TBJ5
Gene ID:
55079
Database links:Entrez Gene: 55079 Human
Entrez Gene: 54713 Mouse
Omim: 607414 Human
SwissProt: Q8TBJ5 Human
SwissProt: Q9ESP5 Mouse
Unigene: 241523 Human
Product Picture Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Spinal cord tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-ZNF312 (SL12148R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF312 Polyclonal Antibody, Unconjugated(SL12148R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF312) Polyclonal Antibody, Unconjugated (SL12148R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF312) Polyclonal Antibody, Unconjugated (SL12148R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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