Product Name	TOP1MT
Chinese Name	MitochondrionDNA拓普西异构酶Ⅰ抗体
Alias	Topoisomerase (DNA) I, mitochondrial; mitochondrial DNA topoisomerase I; DNA topoisomerase I, mitochondrial; TOP1mt; 2900052H09Rik; TOP1MT; TOP1M_HUMAN; DNA topoisomerase I, mitochondrial; mitochondrial; TOP1mt; Topoisomerase I mitochondrial.
literatures	Specific References  (1)     |     SL1211R has been referenced in 1 publications. [IF=11.561] Hangas et al. Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2. (2018) Nucleic.Acids.Res. 46:9625-9636  WB ;  Mouse.   PubMed:30169847
Research Area	Tumour  Chromatin and nuclear signals  transcriptional regulatory factor  Kinases and Phosphatases  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	66kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TOP1mt: 366-488/601
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]. Function: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Subcellular Location: Mitochondrion. Tissue Specificity: Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. Similarity: Belongs to the type IB topoisomerase family. SWISS: Q969P6 Gene ID: 116447 Database links: Entrez Gene: 116447 Human Omim: 606387 Human SwissProt: Q969P6 Human Unigene: 528574 Human
Product Picture	Sample: HepG2 Cell Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-TOP1MT (SL1211R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TOP1MT) Polyclonal Antibody, Unconjugated (SL1211R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TOP1mt/TOPO-I Polyclonal Antibody, Unconjugated(SL1211R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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