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Rabbit Anti-PASD5 antibody
Rabbit Anti-PASD5 antibody
NPAS1; Basic-helix-loop-helix-PAS protein MOP5; bHLHe11; Member of PAS protein 5; MOP5; Neuronal PAS domain protein 1; Neuronal PAS1; NPAS1; NPAS1_HUMAN.
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  • NO.:SL11923R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Rabbit,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name PASD5
Chinese Name 神经元PAS结构域蛋白5抗体
Alias NPAS1; Basic-helix-loop-helix-PAS protein MOP5; bHLHe11; Member of PAS protein 5; MOP5; Neuronal PAS domain protein 1; Neuronal PAS1; NPAS1; NPAS1_HUMAN.  
Research Area Neurobiology  transcriptional regulatory factor  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Rabbit, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 63kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human PASD5/NPAS1: 21-120/590 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of this gene is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. [provided by RefSeq, Jul 2008]

Function:
PASD5 is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of PASD5 is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Interacts with ARNT

Subcellular Location:
Nuclear

Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

SWISS:
Q99742

Gene ID:
4861

Database links:

Entrez Gene: 4861 Human

Omim: 603346 Human

SwissProt: Q99742 Human

Unigene: 79564 Human



Product Picture
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-PASD5 (SL11923R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PASD5 Polyclonal Antibody, Unconjugated(SL11923R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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