Product Name	FEZ2
Chinese Name	椎骨蛋白2抗体
Alias	Zygin2; Fasciculation and elongation protein zeta 2; Fasciculation and elongation protein zeta-2; FEZ 2; Fez2; FEZ2_HUMAN; Highly similar to zyginII; HUM3CL; MGC117372; Pre T/NK cell associated protein; Zygin 2; Zygin II; Zygin-2.
Research Area	Cell biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FEZ2/Zygin2: 165-240/353
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008] Function: Involved in axonal outgrowth and fasciculation. Subunit: Homodimer; difulfide-linked. May form heterodimers with FEZ1. Interacts with synaptotagmin. Tissue Specificity: Expressed in nonneural tissues, such as heart, lung, spleen, muscle, testis, placenta and melanocytes. Similarity: Belongs to the zygin family. SWISS: Q9UHY8 Gene ID: 9637 Database links: Entrez Gene: 9637 Human Entrez Gene: 225020 Mouse Entrez Gene: 94269 Rat SwissProt: Q76LN0 Human SwissProt: Q9UHY8 Human SwissProt: Q6TYB5 Mouse SwissProt: P97578 Rat Unigene: 258563 Human Unigene: 236835 Mouse Unigene: 8714 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- FEZ2 (SL11840R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 46 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (FEZ2) Polyclonal Antibody, Unconjugated (SL11840R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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