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Product Name DOCK7 Chinese Name 胞质分裂专一蛋白7抗体 Alias dedicator of cytokinesis 7; Dedicator of cytokinesis protein 7; Gm430; KIAA1771 ; MGC189434; mKIAA1771; RP23 329P19.2; ZIR2; DOCK7_HUMAN. Research Area Cell biology Neurobiology Signal transduction G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 242kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DOCK7: 1401-1500/2140 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail DOCK 7 is a 2,140 amino acid protein that localizes to developing axons and contains one DHR-1 domain and one DHR-2 domain. Expressed in a variety of tissues, DOCK 7 functions as a guanine nucleotide exchange factor (GEF) that specifically activates Rac 1 and Rac 3 by catalyzing the exchange of bound GDP for free GTP. Multiple isoforms of DOCK 7 exist due to alternative splicing events.
Function:
DOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. It does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization.
Subunit:
Interacts with TSC1. Interacts with nucleotide-free RAC1 and RAC3.
Subcellular Location:
Cell projection,
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the DOCK family.
Contains 1 DHR-1 domain.
Contains 1 DHR-2 domain.
SWISS:
Q96N67
Gene ID:
85440
Database links:Entrez Gene: 85440 Human
GenBank: BC016392.1 Human
SwissProt: Q96N67 Human
Unigene: 406156 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOCK7) Polyclonal Antibody, Unconjugated (SL11825R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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