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Product Name CDKN2A/p19ARF Chinese Name 抑癌基因p19抗体 Alias CD2A2_HUMAN; ARF; Cyclin dependent kinase inhibitor 2A; INK4; MTS1; P19. literatures Specific References (1) | SL1174R has been referenced in 1 publications.[IF=2.33] Liang, Wu, et al. "Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells." Molecular and cellular biochemistry (2013): 1-9. WB ; Human.Research Area Tumour Cell biology Neurobiology Signal transduction Apoptosis Cyclin Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Mouse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization The nucleus Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse CDKN2A/p19ARF: 65-169/169 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD2A2 Polyclonal Antibody, Unconjugated(SL1174R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min;
Incubation: Anti-CD2A2 Polyclonal Antibody, Unconjugated(SL1174R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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