Product Name	ER81
Chinese Name	转录因子ER81蛋白抗体
Alias	DKFZp781L0674; ER81; ER81 protein; ETS translocation variant 1; ets variant gene 1; Ets-related protein 81; Etsrp81; ETV 1; ETV1; ETV1_HUMAN; MGC104699; MGC120533; MGC120534.
Research Area	Cell biology  Neurobiology  Stem cells  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	55kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ER81: 21-120/477
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Several members of the Ets gene family encode sequence-specific DNA binding proteins that recognize DNA sequences with a centrally located 5'-GGAA-3' element. All of the Ets proteins recognize the same central core sequence but each protein interacts with unique sequences that flank this core. PEA3 binds the motif 5'-AGGAAG-3', while ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin. Unlike other members of the Ets family, including Ets-1 and Ets-2, PEA3 is not expressed in hematopoietic cells. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81, PEA3 and ERM display unique expression patterns which suggest these transcriptional factors play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity, while MAPKAP kinase 2 inhibits ER81. Function: Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'. Subcellular Location: Nucleus. Tissue Specificity: Very highly expressed in brain, highly expressed in testis, lung and heart, moderately in spleen, small intestine, pancreas and colon, weakly in liver, prostate and thymus, very weakly in skeletal muscle, kidney and ovary and not in placenta and peripheral blood leukocytes. Post-translational modifications: Sumoylated. DISEASE: Defects in ETV1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ETV1 is found in patients with Erwing sarcoma. Translocation t(7;22)(p22;q12) with EWSR1. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. SWISS: P50549 Gene ID: 2115 Database links: Entrez Gene: 395748 Chicken Entrez Gene: 540846 Cow Entrez Gene: 475246 Dog Entrez Gene: 2115 Human Entrez Gene: 14009 Mouse Entrez Gene: 362733 Rat Omim: 600541 Human SwissProt: Q2KIC2 Cow SwissProt: P50549 Human SwissProt: P41164 Mouse Unigene: 22634 Human Unigene: 4866 Mouse
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-ER81 (SL11745R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ER81 Polyclonal Antibody, Unconjugated(SL11745R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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