Product Name	ROCK1
Chinese Name	Rho相关蛋白激酶1抗体
Alias	p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.
literatures	Specific References  (8)     |     SL1166R has been referenced in 8 publications. [IF=6.831] Yuan-Jing Zou. et al. RAB14 GTPase is essential for actin-based asymmetric division during mouse oocyte maturation. 2021 Jul 29  IF ;  Mouse.   PubMed:34323331 [IF=4.76] Gong, Tao, et al. "Dynamically tunable polymer microwells for directing mesenchymal stem cells differentiation into osteogenesis." Journal of Materials Chemistry B (2015).  WB ;  Rat.   PubMed:doi:10.1039/C5TB01682G [IF=4.15] Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).  WB ;  Rat.   PubMed:23624342 [IF=4.014] Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051  WB ;  Chicken.   PubMed:35961254 [IF=3.923] Pan T et al. MiR-128-1-5p regulates tight junction induced by selenium deficiency via targeting cell adhesion molecule 1 in broilers vein endothelial cells.J Cell Physiol. 2018 Nov;233(11):8802-8814.  WB ;  broiler.   PubMed:29904913 [IF=3.391] Xiaoli Gao. et al. Epigenetic upregulation of ssc-miR-124a following treatment with Clostridium perfringens beta2-toxin attenuates both apoptosis and inflammation in intestinal porcine epithelial cells. Arch Biochem Biophys. 2021 Apr;701:108806  WB ;  Pig.   PubMed:33587903 [IF=2.639] Jiarong Yanget al. Effects of Different Doses of Calcium on the Mitochondrial Apoptotic Pathway and Rho/ROCK Signaling Pathway in the Bone of Fluorosis Rats. Biol Trace Elem Res . 2020 Jul 25.  IHC ;  rat.   PubMed:32710350 [IF=1.735] Bianca Lourdes FREHNER. et al. Implications of the RhoA / Rho associated kinase pathway and leptin in primary uterine inertia in the dog. 2021 Mar 21  IHC ;  Dog.   PubMed:33746146
Research Area	Tumour  Cell biology  Signal transduction  Cyclin  Kinases and Phosphatases  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	158kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ROCK1: 1001-1200/1354
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Subunit: Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes. Tissue Specificity: Detected in blood platelets. Post-translational modifications: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat. SWISS: Q13464 Gene ID: 6093 Database links: Entrez Gene: 6093 Human Entrez Gene: 19877 Mouse Entrez Gene: 81762 Rat Omim: 601702 Human SwissProt: Q8MIT6 Cow SwissProt: Q13464 Human SwissProt: P70335 Mouse SwissProt: Q63644 Rat ROCK1也是丝/苏氨基酸激酶的一种，可调节cell factor，促进平滑肌收缩和肌动蛋白的形成,因此又称：含有卷曲螺旋的蛋白激酶。目前主要用于Tumour浸润、转移方面的研究。
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ROCK1 (SL1166R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 158 kD Observed band size: 160 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- ROCK1 (SL1166R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 160 kD Observed band size: 160 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ROCK1 Polyclonal Antibody, Unconjugated(SL1166R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ROCK1 Polyclonal Antibody, Unconjugated(SL1166R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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