Product Name	PET112L
Chinese Name	细胞色素氧化酶装配因子PET112抗体
Alias	Cytochrome oxidase assembly factor PET112 homolog; GATB_HUMAN; GLU-ADT subunit B; HSPC199; mitochondrial; PET112; PET112 homolog (yeast); PET112-like; Probable glutamyl-tRNA(Gln) amidotransferase subunit B.
Research Area	Cell biology  Signal transduction  Mitochondrion  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	58kDa
Cellular localization	cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PET112L: 101-200/557
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PET112L is the human homolog of the S. cerevisiae COX assembly protein pet112, a protein that is believed to play an important role in the translation of mitochondrial genes. PET112L, also known as HSPC199 or Glu-ADT subunit B (glutamyl-tRNA(Gln) amidotransferase subunit B), is a 557 amino acid protein belonging to the gatB/gatE family of proteins (GatB subfamily) and is believed to play a role in energy metabolism. Localizing to mitochondria, PET112L is expressed in tissues such as heart and muscle, which exhibit high rates of oxidative phosphorylation. The gene encoding PET112L is overexpressed in recurrent ependymoma. Function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Subunit: Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Subcellular Location: Mitochondrion. Tissue Specificity: Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart. Similarity: Belongs to the gatB/gatE family. GatB subfamily. SWISS: O75879 Gene ID: 5188 Database links: Entrez Gene: 5188 Human Omim: 603645 Human SwissProt: O75879 Human Unigene: 119316 Human
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-PET112L (SL11667R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kD Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-PET112L (SL11667R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 58 kD Observed band size: 58 kD

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