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Product Name BHLHB9/p60TRP Chinese Name p60TRP蛋白抗体 Alias Basic helix loop helix domain containing class B 9; BHLHB 9; bHLHB9; KIAA1701; p60 like protein; p60TRP; Protein BHLHb9; Transcription regulator of 60 kDa; BHLH9_HUMAN . literatures Specific References (1) | SL11653R has been referenced in 1 publications.[IF=3.511] Xue Qiao. et al. METTL3/14 and IL-17 signaling contribute to CEBPA-DT enhanced oral cancer cisplatin resistance. 2021 Dec 02 WB,IHC ; Mouse.Research Area Tumour Cell biology Neurobiology Alzheimer's Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 60kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human BHLHB9/p60TRP: 451-547/547 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Basic helix-loop-helix (bHLH) proteins are a group of transcription factors that influence the regulation of neurogenesis, cardiogenesis, myogenesis, differentiation and cell proliferation. p60TRP (p60-transcription-regulator-protein), also known as BHLHb9 (basic helix-loop-helix domain containing, class B, 9) or p60-like protein, is a 547 amino acid cytoplasmic and nuclear protein that belongs to the GPRASP family. A few members of the GRASP family are considered G protein-coupled receptors that play a role in many different stimulus-response pathways. Highly expressed in brain, p60TRP may be involved in the control of cellular aging and survival. In colon cancer cells, p60TRP is down regulated due to CpG hypermethylation of its promoter, and patients suffering from Alzheimer disease have low levels of p60TRP. p60TRP binds to karyopherin ∫3, also known as Ran BP-5, and protein-phosphatase-2A (PP2A), and is encoded by a gene located on human chromosome Xq22.1.
Function:
BHLHB9, or basic helix loop helix domain containing, class B 9, interacts with the Ran-binding-protein-5 (RanBP5) and the protein-phosphatase-2A (PP2A). It can also affect NNT1 and p48ZnF signaling and is down-regulated in the brain of AD subjects. Thus BHLHB9 may have a possible pivotal role in the control of cellular aging and survival. Hypermethylation of the BHLHB9 gene occurs in premalignant lesions and accumulates during tumorigenesis.
Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly cytoplasmic, and nuclear at lower level.
Tissue Specificity:
Highly expressed in brain. Not expressed in lung or liver. Down-regulated in brain from patients suffering from Alzheimer disease.
Similarity:
Belongs to the GPRASP family.
SWISS:
Q6PI77
Gene ID:
80823
Database links:
Product Picture Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BHLHB9/p60TRP Polyclonal Antibody, Unconjugated(SL11653R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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