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Rabbit Anti-HOXC5 antibody
Rabbit Anti-HOXC5 antibody
CP11; homeo box C5; homeobox C5; Homeobox protein CP11; homeobox protein Hox C5; Homeobox protein Hox-3D; Homeobox protein Hox-C5; Hox 3D; HOX3; HOX3D; HOXC5; HXC5_HUMAN; OTTHUMP00000217386.
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  • NO.:SL11589R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,(predicted: Rat,Dog,Cow,Sheep,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name HOXC5
Chinese Name 同源盒蛋白HOXC5抗体
Alias CP11; homeo box C5; homeobox C5; Homeobox protein CP11; homeobox protein Hox C5; Homeobox protein Hox-3D; Homeobox protein Hox-C5; Hox 3D; HOX3; HOX3D; HOXC5; HXC5_HUMAN; OTTHUMP00000217386.  
Research Area Cell biology  Developmental biology  transcriptional regulatory factor  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse,  (predicted: Rat, Dog, Cow, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 25kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human HOXC5: 151-222/222 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The Hox homeobox genes encode proteins that are transcriptional regulators with an established role in embryonic development. HoxC5 is a 222 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. Existing as multiple alternatively spliced isoforms, HoxC5 functions as a sequence-specific DNA-binding transcription factor that is part of a regulatory mechanism that provides cells with positional identities during development. The gene encoding HoxC5 maps to a cluster of Hox proteins on chromosome 12 that are essential for morphogenesis. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12, including hypochondrogenesis, achondrogenesis and Kniest dysplasia.

Function:
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
Q00444

Gene ID:
3222

Database links:

Entrez Gene: 3222 Human

Entrez Gene: 15424 Mouse

Entrez Gene: 315341 Rat

Omim: 142973 Human

SwissProt: Q00444 Human

SwissProt: P32043 Mouse

Unigene: 549040 Human

Unigene: 103825 Mouse

Unigene: 152771 Rat



Product Picture
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti- HOXC5 (SL11589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXC5) Polyclonal Antibody, Unconjugated (SL11589R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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