Product Name	SEMA4G
Chinese Name	Transmembrane proteinSEMA4G抗体
Alias	SEM4G_HUMAN; sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4G; Sema4g; semaphorin 4G; Semaphorin 4G precursor; Semaphorin-4G.
Research Area	Cell biology  Neurobiology  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	90kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SEMA4G: 71-120/838 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA4G (semaphorin-4G) is an 838 amino acid single-pass type I membrane protein that is thought to play a role in axon guidance. Existing as three alternatively spliced isoforms, SEMA4G contains one Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a single SEMA domain. Function: Cell surface receptor for PLXNB2. May play a role in axon guidance. Subunit: Interacts with PLXNB2 Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain. SWISS: Q9NTN9 Gene ID: 57715 Database links: Entrez Gene: 57715 Human SwissProt: Q9NTN9 Human Unigene: 591923 Human Unigene: 735152 Human
Product Picture	Sample: HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- SEMA4G (SL11479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 105kD

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