Product Name	NRAS
Chinese Name	原癌基因N-Ras抗体
Alias	GTPase NRas; OTTMUSP00000023521; ALPS4; AV095280; HRAS1; N ras; N ras protein part 4; Neuroblastoma RAS viral (v ras) oncogene homolog; NRAS1; OTTHUMP00000013879; Transforming protein N Ras; v ras neuroblastoma RAS viral oncogene homolog.
Research Area	Tumour
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human N-Ras: 101-189/189
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	N-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins. Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Subunit: Interacts (active GTP-bound form preferentially) with RGS14. Interacts (active GTP-bound form) with RASSF7. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus. Post-translational modifications: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). DISEASE: Leukemia, juvenile myelomonocytic (JMML) [MIM:607785]: An aggressive pediatric myelodysplastic syndrome/myeloproliferative disorder characterized by malignant transformation in the hematopoietic stem cell compartment with proliferation of differentiated progeny. Patients have splenomegaly, enlarged lymph nodes, rashes, and hemorrhages. Note=The disease is caused by mutations affecting the gene represented in this entry. Noonan syndrome 6 (NS6) [MIM:613224]: A form of Noonan syndrome, a disease characterized by short stature, facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears, and a high incidence of congenital heart defects and hypertrophic cardiomyopathy. Other features can include a short neck with webbing or redundancy of skin, deafness, motor delay, variable intellectual deficits, multiple skeletal defects, cryptorchidism, and bleeding diathesis. Individuals with Noonan syndrome are at risk of juvenile myelomonocytic leukemia, a myeloproliferative disorder characterized by excessive production of myelomonocytic cells. Note=The disease is caused by mutations affecting the gene represented in this entry. Autoimmune lymphoproliferative syndrome 4 (ALPS4) [MIM:614470]: A disorder of apoptosis, characterized by chronic accumulation of non-malignant lymphocytes, defective lymphocyte apoptosis, and an increased risk for the development of hematologic malignancies. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the small GTPase superfamily. Ras family. SWISS: P01111 Gene ID: 4893 Database links: Entrez Gene: 4893 Human Entrez Gene: 18176 Mouse Entrez Gene: 24605 Rat Omim: 164790 Human SwissProt: P01111 Human SwissProt: P08556 Mouse SwissProt: Q04970 Rat Unigene: 486502 Human Unigene: 400954 Mouse Unigene: 217722 Rat 细胞Signal transduction系统紊乱是Tumour细胞生长的重要特征之一，Ras蛋白参与体内多种细胞Signal transduction途径而发挥作用,而原癌基因N-ras是一种多功能的cell factor，广泛存在于自然界，N-ras在多种细胞生命活动中起极为重要的作用，包括细胞的增殖、分化和Cytoskeleton的构建等等，该抗体主要用于Tumour方面的研究。
Product Picture	Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated(SL1146R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human gastric carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated(SL1146R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(SL0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.

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