Product Name	Ephexin-1
Chinese Name	神经细胞鸟苷酸置换因子NGEF抗体
Alias	ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	82kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EPHEXIN: 451-550/710
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species. Function: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension. Subunit: Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase Subcellular Location: Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones. Tissue Specificity: Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. Post-translational modifications: Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain. SWISS: Q8N5V2 Gene ID: 25791 Database links: Entrez Gene: 25791 Human Entrez Gene: 53972 Mouse Entrez Gene: 246217 Rat Entrez Gene: 424744 Chicken Omim: 605991 Human SwissProt: Q8N5V2 Human SwissProt: Q8CHT1 Mouse SwissProt: Q5BKC9 Rat Unigene: 97316 Human Unigene: 435439 Mouse Unigene: 20670 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ephexin-1) Polyclonal Antibody, Unconjugated (SL11458R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Positive control: RSC96 Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μl in 100 μL1X PBS containing 0.5% BSA.

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