Product Name	ZNF318
Chinese Name	Zinc finger protein318抗体
Alias	Endocrine Regulatory Protein; HGNC13578; HRIHFB2436; ZFP318; Zinc Finger Protein 318; ZN318_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  Endocrinopathy  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	251kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ZNF318: 1077-1125/2279
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ZNF318 is a 2279 amino acid endocrine regulatory protein that localizes to the nucleus. Highly expressed in testis, ovaries and kidneys, ZNF318 is a co-repressor of androgen receptor (AR)-mediated transcriptional activation and is thought to regulate transcription during spermatogenesis. ZNF318 interacts with the N-terminal domain of AR and contains two matrin-type zinc fingers. Two isoforms of ZNF318, designated TZF and TZF-L, are produced due to alternative splicing events. Each of these splice variants are thought to have unique roles in transcriptional regulation. While the TZF isoform functions as a repressor of AR-mediated transcriptional activation, the TZF-L isoform is thought to enhance AR-mediated transcriptional activation. Function: ZNF318 encodes a nuclear protein with a zinc finger motif of the Cys2-His2 type that is a novel corepressor of androgen receptor. Subunit: Interacts with the N-terminal domain of AR. Subcellular Location: Nuclear. Tissue Specificity: Expressed in endocrine tissue. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 2 matrin-type zinc fingers. SWISS: Q5VUA4 Gene ID: 24149 Database links: Entrez Gene: 24149 Human SwissProt: Q5VUA4 Human SwissProt: Q99PP2 Mouse Unigene: 509718 Human
Product Picture	Sample: Lymph node(Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug A549(Human) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- ZNF318 (SL11414R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kD Observed band size: 125 kD Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF318) Polyclonal Antibody, Unconjugated (SL11414R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 3

  3Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• E

  EDelete

• 
• E

  EDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout