Product Name	RNF39
Chinese Name	Ring finger protein39抗体
Alias	HZF; HZFW; HZFW1; LIRF; Protein HZFw; RING finger protein 39; Rnf39; RNF39_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  Ring finger protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Monkey, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	38/45kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RNF39: 182-230/420
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF39 (RING finger protein 39), also known as HZFW, HZF or LIRF, is a 420 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger and one SPRY domain. Expressed in testis, RNF39 is thought to play a role in maintaining prolonged LTP (long term-potentiation, or the process by which synaptic strength continues to increases following chemical stimulation). Via its ability to influence the length of the LTP response, RNF39 functions to regulate early synaptic plasticity. Multiple isoforms of RNF39 exist due to alternative splicing events. Function: May play a role in prolonged long term-potentiation (LTP) maintenance. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in testis. Similarity: Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q9H2S5 Gene ID: 80352 Database links: Entrez Gene: 80352 Human Entrez Gene: 171387 Rat Omim: 607524 Human SwissProt: Q9H2S5 Human SwissProt: Q920M2 Rat Unigene: 121178 Human Unigene: 144662 Rat
Product Picture	Protein: Raji lysate at 40ug; Primary: rabbit Anti-RNF39 (SL11393R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size: 38/45 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (SL11393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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