Product Name	A2LD1
Chinese Name	A2LD1蛋白抗体
Alias	A2ld1; GGACT_HUMAN; AIG2-like domain-containing protein 1; Gamma-glutamylaminecyclotransferase; GGACT.
Research Area	Tumour  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human A2LD1: 1-50/153
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	A2LD1 is a 153 amino acid protein belonging to the gamma-glutamylcyclotransferase family. A2LD1 contains two exons, with only the second exon coding, and spans 2.2 kb. Consisting of a monomer subunit, A2LD1 participates in acyltransferase and gamma-glutamylcyclotransferase activities. A2LD1 assists with the breakdown of proteins cross-linked by transglutaminases. A2LD1 also catalyzes the formation of 5-oxoproline from L-gamma-glutamyl-L-epsilon-lysine, but is inactive with L-gamma-glutamyl-alpha-amino acid substrates, such as L-gamma-glutamyl-L-alpha-cysteine and L-gamma-glutamyl-L-alpha-alanine. A2LD1 is encoded by a gene that maps to human chromosome 13q32.3. Function: Contributes to degradation of proteins cross-linked by transglutaminases. Degrades the cross-link between a lysine and a glutamic acid residue from two proteins that have been cross-linked by transglutaminases. Catalyzes the formation of 5-oxoproline from L-gamma-glutamyl-L-epsilon-lysine. Inactive with L-gamma-glutamyl-alpha-amino acid substrates such as L-gamma-glutamyl-L-alpha-cysteine and L-gamma-glutamyl-L-alpha-alanine. Subunit: Monomer. Similarity: Belongs to the gamma-glutamylcyclotransferase family. SWISS: Q9BVM4 Gene ID: 87769 Database links: Entrez Gene: 87769 Human Omim: 613378 Human SwissProt: Q9BVM4 Human Unigene: 350868 Human
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- A2LD1 (SL11381R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (A2LD1) Polyclonal Antibody, Unconjugated (SL11381R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-A2LD1 Polyclonal Antibody, Unconjugated(SL11381R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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