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Product Name Syntaxin 13 Chinese Name 神经突触素13抗体 Alias Syntaxin13; Syntaxin 13; Syntaxin-13; Syx13; Syx 13; Syx-13; CG11278; CT31479; Dm Syx13; ff16; l(3)01470; STX12_RAT. literatures Specific References (1) | SL11378R has been referenced in 1 publications.[IF=4.4] Otero et al. Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function. (2014) J.Cell.Sci. 127:1537-49 WB ; Mouse.Research Area Cell biology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from rat Syntaxin 13: 101-200/274 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Syntaxin 13 is an integral membrane protein that belongs to the t-SNARE family, a group of proteins involved in protein transport. Confocal immunofluoresence and electron microscopy studies have shown that syntaxin 13 is primarily localized to tubular early and recycling endosomes, where it colocalizes with transferrin receptor, and it is also localized in endosomal vacuoles. Syntaxin 13 has been found to be expressed in all tissues, with higher levels of the protein found in brain, lung, spleen, thymus and testes. Immunoprecipitation studies show that syntaxin 13 complexes with beta-SNAP, VAMP2/3, and SNAP25. The binding of this complex to alpha-SNAP and NSF is terminated in the presence of ATP. These results suggest that syntaxin 13 is a SNARE protein which mediates the recycling protein flow through tubulo vesicular recycling endosomes.
Function:
SNARE that acts to regulate protein transport between endosomes and the trans-Golgi network (By similarity). The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro).
Subunit:
Associates with the BLOC-1 complex. Interacts with BLOC1S6 (By similarity). Interacts with NAPA and SNAP23. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.
Subcellular Location:
Endosome membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Endomembrane system; Single-pass type IV membrane protein; Cytoplasmic side (Potential).
Tissue Specificity:
Ubiquitous. Highly expressed in brain.
Similarity:
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
SWISS:
G3V7P1
Gene ID:
65033
Database links:
UniProtKB/Swiss-Prot: G3V7P1.1
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syntaxin 13) Polyclonal Antibody, Unconjugated (SL11378R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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