Product Name	RIM2
Chinese Name	神经元突触膜胞外分泌调节蛋白2抗体
Alias	Non small cell lung cancer RimL3a protein; Rab3 interacting molecule 2; RAB3IP3; Regulating synaptic membrane exocytosis protein 2; Rims2; RIMS2_HUMAN.
Research Area	Cell biology  Neurobiology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	Flow-Cyt=3ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	160kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RIM2: 801-900/1411
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Rab3, a neural/neuroendocrine-specific member of the Rab family, is involved in Ca2+-regulated exocytosis (1-2). Rab3 functions in an inhibitory capacity by controlling the recruitment of secretory vesicles into a releasable pool at the plasma membrane. Rim (rab3 interacting molecule), a putative effector protein for Rab3s, is composed of an amino-terminal zinc-finger motif and carboxy-terminal PDZ and C2 domains. Rim exists as two variants, Rim1 and Rim2, produced by alternative splicing (3). Rim1 is expressed near the active zone at the synapse, where it interacts in a GTP-dependent manner with Rab3, located on synaptic vesicles (4). Therefore, Rim serves as a Rab3-dependent regulator of synaptic-vesicle fusion by forming a GTP-dependent complex between synaptic plasma membranes and docked synaptic vesicles (5). Both Rim1 and Rim2 can bind to cAMP-GEFII, which is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-dependent exocytosis (3). Rim also localizes on the plasma membrane of INS-1E cells and pancreatic beta-cells. Rab3 binding domain of Rim enhances glucose-stimulated secretion in intact cells and Ca2+-stimulated exocytosis in permeabilized cells, suggesting that Rim may also play a regulatory role in insulin secretion (6). Function: RIM2 is a rab effector involved in exocytosis. It may act as scaffold protein. It is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP dependent manner. Subunit: Interacts with RAB3A and RAB3B that have been activated by GTP-binding. Interacts with RAB3C, RAB3D and RAB26. Interacts with BZRAP1/RIMBP1 and RIMBP2. Interacts with PPFIA3 and PPFIA4. Interacts via its zinc finger with the first C2 domain of UNC13A. Forms a complex consisting of UNC13A, RIMS2 and RAB3A. Heterodimer with PCLO. Part of a ternary complex involving PCLO and EPAC2 (By similarity). Subcellular Location: Cell membrane; Peripheral membrane protein (By similarity). Cell junction, synapse (By similarity). Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein (By similarity). Similarity: Contains 2 C2 domains. Contains 1 FYVE-type zinc finger. Contains 1 PDZ (DHR) domain. Contains 1 RabBD (Rab-binding) domain. SWISS: Q9UQ26 Gene ID: 9699 Database links: Entrez Gene: 9699 Human Entrez Gene: 116838 Mouse Entrez Gene: 116839 Rat Omim: 606630 Human SwissProt: Q9UQ26 Human SwissProt: Q9EQZ7 Mouse SwissProt: Q9JIS1 Rat Unigene: 655271 Human Unigene: 735969 Human Unigene: 309296 Mouse Unigene: 161948 Rat
Product Picture	Blank control: A431. Primary Antibody (green line): Rabbit Anti-RIM2 antibody (SL11357R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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