Product Name	TBR2
Chinese Name	脱中胚蛋白抗体
Alias	Eomes; EOMES_HUMAN; Eomesodermin; Eomesodermin homolog; t box brain 2; T-box brain protein 2; T-brain-2; Tbr 2; TBR-2.
literatures	Specific References  (1)     |     SL11331R has been referenced in 1 publications. [IF=6.208] Jia Wang. et al. FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice. INT J MOL SCI. 2022 Jan;23(23):14979  IHC, IF ;  Mouse.   PubMed:36499306
Research Area	Cell biology  Developmental biology  Neurobiology  Stem cells  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	73kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TBR2: 301-400/686
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	EOMES (eomesodermin homolog), also known as TBR2 (T-box-brain2), is the 686 amino acid human homolog of the mouse Eomes protein that contains one T-box DNA binding domain. Genes that contain T-box domains encode proteins that function as transcription factors and are often involved in the regulation of various developmental events. Localized to the nucleus and expressed in the developing brain, EOMES is thought to be involved in neuronal migration and division and may play a role in trophoblast development and gastrulation. Silencing of the EOMES gene can cause mutated or arrested development and may lead to microcephaly disorders which are characterized by reduced head circumference and a malformed brain. Function: Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Subcellular Location: Nucleus. Tissue Specificity: Expressed in CD8+ T-cells. DISEASE: Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum. Similarity: Contains 1 T-box DNA-binding domain. SWISS: O95936 Gene ID: 8320 Database links: Entrez Gene: 8320 Human Entrez Gene: 13813 Mouse Omim: 604615 Human SwissProt: O95936 Human SwissProt: O54839 Mouse Unigene: 591663 Human Unigene: 200692 Mouse
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated(SL11331R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-TBR2antibody (SL11331R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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