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Product Name MCM4 Chinese Name 微小染色体维持缺陷蛋白4抗体 Alias CDC21; CDC21 homolog; CDC54; DNA replication licensing factor MCM 4; DNA replication licensing factor MCM4; hCdc 21; hCdc21; Homolog of S. pombe cell devision cycle 21; KIAA4003; mcdc21; MCM 4; MCM 4 minichromosome maintenance deficient 4; MCM4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae); MCM4_HUMAN; Mcmd4; MGC33310; Minichromosome Maintenance 4; Minichromosome maintenance complex component 4; Minichromosome maintenance deficient (S. cerevisiae) 4; Minichromosome maintenance deficient 4; Minichromosome maintenance deficient 4 homolog (S. cerevisiae); Minichromosome maintenance, S. cerevisiae, homolog of, 4; mKIAA4003; P1 CDC21; P1-CDC21; P1CDC21; 19G antibody AI325074; AU045576; CDC 21; CDC 54. Research Area Cell biology Apoptosis Cyclin Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 96kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MCM4: 761-863/863 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis.
Function:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Subunit:
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with MCMBP.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the MCM family.
Contains 1 MCM domain.
SWISS:
P33991
Gene ID:
4173
Database links:Entrez Gene: 4173 Human
Entrez Gene: 17217 Mouse
Omim: 602638 Human
SwissProt: P33991 Human
SwissProt: P49717 Mouse
Unigene: 460184 Human
Unigene: 1500 Mouse
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