Product Name	RAB10
Chinese Name	G蛋白Binding proteinRAB10抗体
Alias	GTP binding protein RAB10; Rab 10; Rab-10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10.
Research Area	Signal transduction  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	23kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RAB10: 41-140/200
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21 (1). Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport (1,2). The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function (1-6). The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles (9). Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway (3-8). Function: The small GTPases Rab are key regulators ofintracellular membrane trafficking, from the formation of transportvesicles to their fusion with membranes. Rabs cycle between aninactive GDP-bound form and an active GTP-bound form that is ableto recruit to membranes different set of downstream effectorsdirectly responsible for vesicle formation, movement, tethering andfusion (By similarity). That Rab is mainly involved in thebiosynthetic transport of proteins from the Golgi to the plasmamembrane. Regulates, for instance, SLC2A4/GLUT4 glucosetransporter-enriched vesicles delivery to the plasma membrane. Inparallel, it regulates the transport of TLR4, a toll-like receptorto the plasma membrane and therefore may be important for innateimmune response. Plays also a specific role in asymmetric proteintransport to the plasma membrane within the polarized neuron andepithelial cells. In neurons, it is involved in axonogenesisthrough regulation of vesicular membrane trafficking toward theaxonal plasma membrane while in epithelial cells, it regulatestransport from the Golgi to the basolateral membrane. Moreover, mayplay a role in the basolateral recycling pathway and in phagosomematuration. According to PubMed:23263280, may play a role inendoplasmic reticulum dynamics and morphology controllingtubulation along microtubules and tubules fusion. [ENZYME REGULATION] Rab activation is generally mediated by aguanine exchange factor (GEF), while inactivation throughhydrolysis of bound GTP is catalyzed by a GTPase activating protein(GAP) (By similarity). That Rab is activated by the DENND4C guanineexchange factor (GEF). Subunit: Interacts with MYO5A; mediates the transport to theplasma membrane of SLC2A4/GLUT4 storage vesicles. Interacts withGDI1 and maybe with GDI2; negatively regulates RAB10 associationwith membranes and activation. Interacts (GDP-bound form) withLLGL1; the interaction is direct and promotes RAB10 associationwith membranes and activation through competition with the Rabinhibitor GDI1 (By similarity). Interacts with EXOC4; probablyassociates with the exocyst (By similarity). Subcellular Location: Cytoplasmic vesicle membrane; Lipid-anchor(Probable); Cytoplasmic side (Probable). Golgi apparatus,trans-Golgi network membrane (By similarity). Endosome membrane.Recycling endosome membrane. Cytoplasmic vesicle, phagosomemembrane (By similarity). Cell projection, cilium. Endoplasmicreticulum membrane. Note=Associates with SLC2A4/GLUT4 storagevesicles. Localizes to the base of the cilium. Transientlyassociates with phagosomes (By similarity). According toPubMed:23263280 localizes to the endoplasmic reticulum at domainsof new tubule growth. DISEASE: Belongs to the small GTPase superfamily. Rab family. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: P61026 Gene ID: 10890 Database links: Entrez Gene: 421994 Chicken Entrez Gene: 403958 Dog Entrez Gene: 19325 Mouse Entrez Gene: 50993 Rat SwissProt: P24409 Dog SwissProt: P61027 Mouse SwissProt: P35281 Rat Unigene: 378993 Mouse Unigene: 486858 Mouse Unigene: 65864 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB10) Polyclonal Antibody, Unconjugated (SL11257R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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