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Product Name Calcineurin B Chinese Name 钙调磷酸酶B亚基B1抗体 Alias Calcineurin subunit B type 1; PP2B-B1; CALNB1; CANB1_HUMAN; Cna2; CNB; CNB1; OTTHUMP00000201960; OTTHUMP00000201961; Ppp3r1; PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; alpha isoform (calcineurin B, type I); calcineurin B, type I (19kDa); protein phosphatase3 (formerly2B), regulatory subunit B, alpha isoform antibody Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); Protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1. Research Area Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Calcineurin B/PP2B-B1: 31-130/170 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-A alpha, PP2B-A Beta and PP2B-A Gamma. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.
Function:
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Subunit:
Composed of a catalytic subunit (A) and a regulatory subunit (B).
Similarity:
Belongs to the calcineurin regulatory subunit family. Contains 4 EF-hand domains.
SWISS:
Q96LZ3
Gene ID:
5534
Database links:Entrez Gene: 5534 Human
Entrez Gene: 19058 Mouse
Omim: 601302 Human
SwissProt: P63098 Human
SwissProt: Q63810 Mouse
Unigene: 280604 Human
Unigene: 41840 Mouse
Unigene: 42903 Rat
Product Picture Sample:HL-60 (Human)Cell Lysate at 40 ug
Primary: Anti-Calcineurin B(SL11246R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 19kD
Observed band size: 19kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calcineurin B) Polyclonal Antibody, Unconjugated (SL11246R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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