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Product Name TCF12 Chinese Name 转录因子12抗体 Alias bHLHb20; Class B basic helix-loop-helix protein 20; DNA binding protein HTF4; DNA-binding protein HTF4; E box binding protein; E-box-binding protein; HEB; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4. literatures Specific References (2) | SL11237R has been referenced in 2 publications.[IF=5.546] Mengjin Gao. et al. circHIPK3 regulates proliferation and differentiation of myoblast through the miR‐7/TCF12 pathway. 2021 Mar 21 WB ; Mouse.[IF=3.65] Chen, Qing-biao, et al. "Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer." Tumor Biology 39.6 (2017): 1010428317703924. WB, IHC-P ; Human.Research Area transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Horse, Rabbit, Zebrafish, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 73kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TCF12: 51-150/682 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression.
Function:
Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems.
Subunit:
Contains 1 basic helix-loop-helix (bHLH) domain.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
Similarity:
Contains 1 bHLH (basic helix-loop-helix) domain.
SWISS:
Q99081
Gene ID:
6938
Database links:Entrez Gene: 6938 Human
Omim: 600480 Human
SwissProt: Q99081 Human
Unigene: 511504 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (TCF12) Polyclonal Antibody, Unconjugated (SL11237R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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