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Product Name Nfasc186 Chinese Name 神经束蛋白186抗体 Alias Neurofascin; NF antibody NF186; NFASC; NRCAML; NFASC_HUMAN. Research Area Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 147kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Nfasc186 : 501-600/1347 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat; the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons.
Function:
Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions (By similarity).
Subunit:
Horseshoe-shaped homodimer. Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Interacts with GLDN/gliomedin (By similarity).
Subcellular Location:
Cell Membrane; Type I membrane protein.
Similarity:
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
O94856
Gene ID:
23114
Database links:Entrez Gene: 23114 Human
Entrez Gene: 269116 Mouse
SwissProt: O94856 Human
SwissProt: Q810U3 Mouse
Unigene: 13349 Human
Unigene: 326702 Mouse
Unigene: 3048 Rat
Product Picture Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-Nfasc186 (SL11214R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132 kD
Observed band size: 132 kD
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