TEL: +86 571 56623320 EMAIL: [email protected]
Product Name MOBP Chinese Name 少突胶质细胞髓鞘相关蛋白抗体 Alias MGC87379; Myelin associated oligodendrocyte basic protein; MOBP_HUMAN. literatures Specific References (1) | SL11184R has been referenced in 1 publications.[IF=2.161] Kon T et al. Immunoreactivity of myelin‐associated oligodendrocytic basic protein in Lewy bodies. Neuropathology. 2019. WB,IHC-P&IHF ; Human,mouse&rat.Research Area Cell biology Neurobiology Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 21kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MOBP: 7-90/183 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The gene encoding MOBP a member of the central nervous system myelin-constituting proteins, maps to chromosome 3p22.2. MOBP has many splice variants that share a 68 amino acid N-terminal domain. MOBP-71, MOBP-81A, MOBP-99, and MOBP-169 are MOBP splice variants that contain exon 8b, which is similar to myelin basic protein (MBP) mRNA RTS, however MOBP-69, MOBP-81B, and MOBP-170 lack this exon. The splice variants that contain exon 8b are expressed in myelin, while those lacking exon 8b are retained in the oligodendrocyte soma. Exon 8b-containing variants are directed to sites of myelin sheath assembly by exon 8b, where they play a structural role in myelin formation. Splice variants lacking exon 8b likely play a cellular and/or regulatory role. MOBP is implicated in multiple sclerosis (MS), a human demyelinating disease, and in allergic encephalomyelitis in rodents.
Function:
MOBP (Myelin-associated oligodendrocytic basic protein) is abundantly expressed specifically in oligodendrocytes and is the third most abundant protein in CNS myelin. MOBP is present in the major dense line of CNS myelin suggesting a role in the compaction or stabilization of myelin.
Subcellular Location:
Cytoplasm, perinuclear region. Note=Present in the major dense line of CNS myelin (By similarity).
SWISS:
Q13875
Gene ID:
4336
Database links:Entrez Gene: 4336 Human
Entrez Gene: 17433 Mouse
Omim: 600948 Human
SwissProt: Q13875 Human
SwissProt: Q9D2P8 Mouse
Unigene: 121333 Human
Product Picture Positive sample:Paraformaldehyde-fixed, paraffin embedded (rat brian); Negative sample:Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (SL11184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated (SL11184R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Scan Wechat Qrcode