Product Name	IQGAP1
Chinese Name	支架蛋白抗体
Alias	IQ motif containing GTPase activating protein 1; KIAA0051; p195; Ras GTPase activating like protein 1; IQGA1_HUMAN.
literatures	Specific References  (2)     |     SL1109R has been referenced in 2 publications. [IF=5.942] Sheen YS et al. Purpuric drug eruptions induced by EGFR tyrosine kinase inhibitors are associated with IQGAP1‐mediated increase in vascular permeability. J Pathol. 2020 Feb 7.  ICF ;  Human.   PubMed:32030757 [IF=4.67] Fang, Jing, et al. "PAX6 down-regulates miR-124 expression to promote cell migration during embryonic stem cell differentiation." Stem Cells and Development (2014).  Mouse.   PubMed:24773074
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat, Rabbit,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	189kDa
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IQGAP1: 1451-1550/1657
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IQGAP1 is a widely expressed protein than interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology. It binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Function: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Subunit: Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Subcellular Location: Cell membrane. Tissue Specificity: Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. Post-translational modifications: Phosphorylation of Ser-1443 by PKC/PRKCE prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. Similarity: Contains 1 CH (calponin-homology) domain. Contains 4 IQ domains. Contains 1 Ras-GAP domain. Contains 1 WW domain. SWISS: P46940 Gene ID: 8826 Database links: Entrez Gene: 8826 Human Entrez Gene: 29875 Mouse Entrez Gene: 361598 Rat Omim: 603379 Human SwissProt: P46940 Human SwissProt: Q9JKF1 Mouse Unigene: 430551 Human Unigene: 207619 Mouse Unigene: 106939 Rat 支架蛋白IQGAP1与许多重要的细胞活动紧密相关，如黏附和迁移。研究发现在损伤修复、组织发展、Tumour转移等过程中，IQGAP1的表达和功能有明显改变。同时作为Rh.GTPase信号的主要效应因子，IQGAP1能传递细胞外信号至黏附素复合体、Cytoskeleton以及The nucleus。通过上述信号通路，IQ-GAP1协助细胞完成一系列重要生理病理活动。
Product Picture	Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(SL1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rabbit placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IQGAP1 Polyclonal Antibody, Unconjugated(SL1109R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-IQGAP1 antibody (SL1109R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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