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Product Name NCAM2 Chinese Name 神经Cell adhesion molecule2抗体 Alias N CAM 2; N CAM 21; N CAM2; N CAM21; N-CAM-2; NCAM 2; NCAM 21; NCAM-2; Ncam2; NCAM2_HUMAN; NCAM21; Neural cell adhesion molecule 2; OCAM GPI; R4B12; RB 8 neural cell adhesion molecule; RB8 neural cell adhesion molecule; RNCAM. Research Area Neurobiology Signal transduction Cell adhesion molecule The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Rabbit, ) Applications ELISA=1:5000-10000 Flow-Cyt=3ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 91kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NCAM2: 51-150/837 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NCAM2 is an 837 amino acid protein encoded by the human gene NCAM2. NCAM2 contains five immunoglobulin-like domains, two Fibronectin type III domains, a transmembrane domain and a cytoplasmic domain. The gene is expressed most strongly in human adult and fetal brain. NCAM2 is a member of the neural cell adhesion molecule (NCAM) family. NCAMs are closely related cell surface glycoproteins involved in cell to cell interactions during growth and are thought to play an important role in embryogenesis and development. NCAM2 is a considered a good candidate for involvement in certain Down syndrome phenotypes because a slight overexpression of NCAMs increases many-fold the homotypic adhesion properties of cells. Stat5 regulates NCAM2 in vivo by binding to the NCAM2 intron in the NKL natural killer cell line; this binding is induced by cytokines that activate Stat5. Neither Stat1 nor Stat3 bind to this region, despite sharing a consensus binding sequence with Stat5.
Function:
May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
Subunit:
Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Subcellular Location:
Cell membrane.
Tissue Specificity:
Expressed most strongly in adult and fetal brain.
Similarity:
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
O15394
Gene ID:
4685
Database links:Entrez Gene: 4685 Human
Entrez Gene: 17968 Mouse
Omim: 602040 Human
SwissProt: O15394 Human
SwissProt: O35136 Mouse
Unigene: 473450 Human
Unigene: 433941 Mouse
Unigene: 138756 Rat
Product Picture Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-NCAM2 antibody (SL11094R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-NCAM2 antibody (SL11094R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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