Product Name	Optimedin
Chinese Name	嗅球蛋白3抗体
Alias	Noe3; NOE3_HUMAN; Noelin 3; Noelin-3; NOELIN3; NOELIN3 V1; NOELIN3 V2; NOELIN3 V3; NOELIN3 V4; NOELIN3 V5; NOELIN3 V6; Olfactomedin 3; Olfactomedin related ER localized protein 3; Olfactomedin-3; Olfactomedin3; Olfm3; Optimedin.
Research Area	Neurobiology  Signal transduction  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OLFM3/Optimedin: 101-200/478
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	OLFM3 is a 478 amino acid protein that interacts with myocilin. Myocilin is an extracellular protein that plays a key role in the actomyosin system and is responsible for controlling intraocular pressure. OLFM3 is a secreted protein that contains an olfactomedin-like (OLF) domain, an approximately 260 amino acid motif commonly found in secreted glycoproteins. OLFM3 localizes to the Golgi apparatus of the cell and is highly expressed in both eye and brain tissue. Mutations in the gene that encodes OLFM3 may cause severe glaucoma, a condition in which increased intraocular pressure within the eyeball causes loss of eye sight. Subunit: Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including OLFM3. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Homodimer. Interacts with MYOC (By similarity). Subcellular Location: Secreted. Tissue Specificity: In the eye, expressed in trabecular meshwork and neural retina; in non-ocular tissues, expressed in brain and lung. Similarity: Contains 1 olfactomedin-like domain. SWISS: Q96PB7 Gene ID: 118427 Database links: Entrez Gene: 118427 Human Entrez Gene: 229759 Mouse Entrez Gene: 252920 Rat Omim: 607567 Human SwissProt: Q96PB7 Human SwissProt: P63056 Mouse SwissProt: P63057 Rat Unigene: 484475 Human Unigene: 54183 Mouse Unigene: 27711 Rat
Product Picture	Sample: Eye (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- Optimedin (SL11061R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Optimedin) Polyclonal Antibody, Unconjugated (SL11061R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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